Analytical Ultracentrifugation (AUC)

Analytical ultracentrifugation (AUC) is a quantitative technique that determines the size, molecular weight, shape and self-associative properties of macromolecules and nanoparticles in aqueous solution. Combined with the AU-FDS fluorescence system, AUC enables the detection of particle sedimentation in mixtures and complex solutions.

Sirion-SEM_128

 

Further Reading:

Brown PH, Balbo A. Schuck P. 2009, On the analysis of sedimentation velocity in the study of protein complexes, Eur. Biophys J, 38:1079-1099

Casassa EF, Eisenberg H. 1964, Thermodynamic analysis of multicomponent systems, Adv. Prot. Science, Part 1

Demler B, Saber H, Hansen JC. 1997, Identifcation and interpretation of complexity in sedimentation velocity boundaries, 72:397-407

Laue TM, Stafford WF. 1999, Modern applications of analytical ultracentrifugation, Annu. Rev. Biophys. Biomol. Struct. 28:75-100

Schuck Peter, 2000, Size Distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and Lamm equation modeling, Biophysical Journal, 78:1606-1619

Stafford WF, Sherwood PJ. 2004, Analyis of hetorlogous interacting systems by sedimentation velocity: curve fitting algorithms for estimation of sedimentation coefficients, equilibrium and kinetic constants, Biophysical Chemistry, 108:231-243