Biacore T100 Surface Plasmon Resonance (Biacore)
The Biacore T100 is a Surface Plasmon Resonance (SPR) based biosensor for detecting, characterizing and quantifying bimolecular interactions. The Biacore employs a modified gold surface on which a ligand is immobilized. The sample analyte is then injected over this surface using an automated microfluidics system. Binding results in an increase in mass on the surface which is recorded as a change in instrument response over time. This technique has become the gold standard for analyzing biomolecular interactions important in the area of drug discovery and development. SPR technology is a label-free technique, and therefore avoids problems associated with the modification of the protein amino acid sequence or its chemical environment. The high sensitivity and automated sample handling of the T100 makes it ideal for high-throughput biophysical analysis, and the peltier driven temperature control of the system permits biophysical measurements under physiologically relevant conditions. This is a robust technique, amenable to a wide range of samples: proteins, nucleic acids, carbohydrates, lipids, LMW compounds, whole cells, liposomes, viruses and bacteria.